PhytophthoraV1, Main, Exploration, bibRecord, 001008

Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans.

Identifieur interne : 001008 ( Main/Exploration ); précédent : 001007; suivant : 001009

Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans.

Auteurs : Harold J G. Meijer [Pays-Bas] ; Francesco M. Mancuso [Espagne] ; Guadalupe Espadas [Espagne] ; Michael F. Seidl [Pays-Bas] ; Cristina Chiva [Espagne] ; Francine Govers [Pays-Bas] ; Eduard Sabid [Autriche]

Source :

Molecular & cellular proteomics : MCP [ 1535-9484 ] ; 2014.

RBID : pubmed:24872595

Descripteurs français

KwdFr :
- Bases de données de protéines (MeSH), Milieux de culture (composition chimique), Paroi cellulaire (métabolisme), Phosphorylation (MeSH), Phytophthora infestans (composition chimique), Phytophthora infestans (métabolisme), Protéines de protozoaire (isolement et purification), Protéines de protozoaire (métabolisme), Protéomique (méthodes), Spectrométrie de masse (MeSH).
MESH :
- composition chimique : Milieux de culture, Phytophthora infestans.
- isolement et purification : Protéines de protozoaire.
- métabolisme : Paroi cellulaire, Phytophthora infestans, Protéines de protozoaire.
- méthodes : Protéomique.
- Bases de données de protéines, Phosphorylation, Spectrométrie de masse.

English descriptors

KwdEn :
- Cell Wall (metabolism), Culture Media (chemistry), Databases, Protein (MeSH), Mass Spectrometry (MeSH), Phosphorylation (MeSH), Phytophthora infestans (chemistry), Phytophthora infestans (metabolism), Proteomics (methods), Protozoan Proteins (isolation & purification), Protozoan Proteins (metabolism).
MESH :
- chemical , chemistry : Culture Media.
- chemistry : Phytophthora infestans.
- chemical , isolation & purification : Protozoan Proteins.
- metabolism : Cell Wall, Phytophthora infestans, Protozoan Proteins.
- methods : Proteomics.
- Databases, Protein, Mass Spectrometry, Phosphorylation.

Abstract

Oomycetes are filamentous organisms that cause notorious diseases, several of which have a high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes, and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focused on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyze P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses, and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment.

DOI: 10.1074/mcp.M113.035873
PubMed: 24872595
PubMed Central: PMC4125740

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 001053
to stream Main, to step Curation: 001053

Le document en format XML

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<front><div type="abstract" xml:lang="en">Oomycetes are filamentous organisms that cause notorious diseases, several of which have a high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes, and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focused on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyze P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses, and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment. </div>
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<Abstract><AbstractText>Oomycetes are filamentous organisms that cause notorious diseases, several of which have a high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes, and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focused on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyze P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses, and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment. </AbstractText>
<CopyrightInformation>© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.</CopyrightInformation>
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<Initials>FM</Initials>
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<ForeName>Guadalupe</ForeName>
<Initials>G</Initials>
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<Author ValidYN="Y"><LastName>Chiva</LastName>
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Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans.

Profiling the secretome and extracellular proteome of the potato late blight pathogen Phytophthora infestans.

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